VSG Pacbio preprint

It’s been a long time in the making, but Sid’s work on using long read sequencing to determine expressed antigen diversity in Trypanosoma brucei infections has finally been posted on bioRxiv!

In this collaboration with Liam Morrison, we applied long read sequencing (PacBio) to VSG amplicons generated from blood extracted from mice infected with T. brucei. We found that long read sequencing is reliable for resolving allelic differences between VSGs, that there is significant expressed diversity (449 VSGs detected across 20 mice) and that there is a striking semi-reproducible pattern of expressed diversity across the timeframe of study.

Well done Sid and everyone else who contributed to this study!